Tag Archives: fox

What’s In A Wolf Scat? New DNA-Based Method To Detect Prey From Carnivore Scats

This is a guest post by Cecilia di Bernardi.

Image Credit: Rick Heeres, CC BY 2.0, Image Cropped

Multiple species-specific molecular markers using nanofluidic array as a tool to detect prey DNA from carnivore scats (2021) Di Bernardi et al., Ecology & Evolution. https://doi.org/10.1002/ece3.7918

The Crux

Studying carnivore diet can be a crucial tool to inform both management and conservation of predators and their prey. If we’re going to ensure a carnivore’s survival, we need to know which species it relies on for food, and in what quantities.

Digging into an animal’s stomach isn’t the nicest way to get the crucial data we’re looking for, so non-invasive sampling of scats (that’s science for poop) has for been a more ideal approach to collecting valuable information on the occurrence, genetics, and diet of animals, especially when dealing with elusive and threatened species. Nowadays, DNA-based analyses of scats are allowing researchers to get more and more high-resolution data on predators’ food habits.

What We Did

We developed a DNA-based method to detect prey from wolf scats, taking advantage of the huge leaps the DNA analysis has been through in recent years. We also made use of nanotechnology (specifically Nanofluidic array technology fromFluidigm Inc.), which has been useful for detecting pathogen species in ticks, or traces of herbivores on browsed twigs, but has never applied to detect prey from predator scats!

Starting from the big bank of DNA sequences available online (GenBank, NCBI), we looked at specific areas of the genome, (the mitochondrial genome), in order to tell apart the different target prey species present in the wolf scat. We developed species-specific molecular markers (see Did You Know?) and tested them with reference tissue samples, kindly provided by the Swedish Museum of Natural History. After the protocol development and optimization, we ended up with a set of 80 markers for our 18 target species. We then applied the newly developed molecular method on a pilot sample of wolf scats collected in the field.

Did You Know: Molecular Markers

Since any species’ genome is an incredibly long sequence, scientists have developed more efficient ways of defining what DNA belongs to which species. The motivation is simple – if you’re trying to tell whether a genome belongs to a human or to a chimpanzee, you don’t want to be looking through the 99% of DNA we have in common, you want to go straight to that 1%. That’s why scientists develop ‘markers’. It helps them narrow down their search and identify species much more quickly.

What We Found

The molecular markers we developed did their job well, correctly detecting the 18 prey species, showing an overall good distinction between the tissue samples of the target and non-target species. In other words, this means that a tissue sample taken from a moose was detected by the moose markers but not by the reindeer markers, which is the sign of a successful marker!

When applied to the pilot of wolf scats collected in the wild, the method detected a total of 16 species, comprising wild ungulates (moose, roe deer, red deer, fallow deer, wild boar), domestic and semi-domestic animals (reindeer, cattle, sheep), small prey species (European badger, European hare, mountain hare, Western capercaillie, black grouse), and other carnivores (Eurasian lynx, wolverine, red fox).

Just because fox DNA turns up in a wolf scat, it doesn’t mean that a wolf has eaten a fox – it could simply mean tha a fox has urinated on the scat! (Image Credit: Joanne Redwood, CC0 1.0)

Problems?

While the method detects the target species as we’d like, it cannot distinguish whether predation, scavenging, or territorial marking has occurred. Detection of fox DNA in wolf scats can mean a wolf predating on a fox, a wolf scavenging on a fox, but also a fox marking with its urine on a wolf scat! To partly disentangle this aspect, we are investigating the contribution of scavenging to wolves diet in Scandinavia, with data from GPS-collared wolves.

So What?

This molecular method, with its high-resolution prey detection, can help better understanding under what circumstances wolves eat certain prey and how that can affect ungulate populations, serving as a valuable complement to the current GPS technology used to investigate wolf predation. Wolf natural expansion is an ongoing and controversial phenomena in the Northern hemisphere, and any technique that tells us more about their impact is a welcome addition to our knowledge base.


Cecilia Di Bernardi is an ecologist who is currently investigating wolf predation ecology within her PhD at the University of Rome La Sapienza in collaboration with SLU Swedish University of Agricultural Sciences. You can follow her on Twitter @c_dibernardi.

Of Foxes And Wolves, or, The Lady Who Threw Her Dog In A Bin

Image Credit: Dirk-Jan van Roest, CC BY 2.0, Image Cropped

Many parliamentary debates in Norway cover ground that is familiar to other countries; climate change, the economy, pandemic responses. Yet I’m happy to say there’s one issue that is more unique to this part of the world: what to do with all these wolves. Once native to Norway, wolves, bears and wolverines were largely pushed out of the country, but started to come back into parts of Norway in the 1970s after they became protected. Despite what one of Liam Neeson’s better old-man-action films would have you believe, wolves are shy but curious creatures and of no real danger to people. However their reintroduction has generally been met with a mix of both consternation and celebration, with urban populations cheering the reintroduction of a former native and rural populations wary of the thought of sharing space with wolves.

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Can A Harsh Climate Create Stronger Interactions Between Species?

Bowler et al. (2020) Impacts of predator-mediated interactions along a climatic gradient on the population dynamics of an alpine bird. Proceedings of the Royal Society B, 287, https://doi.org/10.1098/rspb.2020.2653.

The Crux

Whether or not a species will survive in an area can usually be broken down into two broad categories: how suitable the environmental characteristics of that area are (temperature, elevation, rainfall), and how it interacts with the other species found nearby. Early ecological theory predicted that in harsh environments, how a species interacts with other species wouldn’t matter as much, and would only come into play when the area was easier for the species to inhabit.

Yet more modern work often contradicts this theory. For instance, the Alternative Prey Hypothesis (APH) suggests that in areas where there are relatively few species as a result of harsh climates, interactions between those few species will be relatively strong. For example, if a prey species declines one year, then its usual predator must find an alternative prey species. This creates an indirect interaction between the two prey species, which is particularly strong in harsh environments where there aren’t other species around.

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Predicting Apocalypses: Lessons From Fox News on the Climate Change Debate

Whilst making people aware of the consequences of climate change and land fragmentation is important, choosing how to deliver that message is equally important (Image Credit: Backbone Campaign, CC BY 2.0, Image Cropped)

Ok, first up, I want to apologise. I know that giving Fox News any attention when it comes to scientific progress is a bad start. I’m hoping that if you’re reading this, you already know that their stance on climate change and biological degradation is… let’s say flawed.

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Why Warmer Winters Don’t Always Help Geese

Image Credit: MaxPixel, CC0 1.0, Image Cropped

Contrasting consequences of climate change for migratory geese: Predation, density dependence and carryover effects offset benefits of high-arctic warming (2019) Layton-Matthews et al., Global Change BiologyDOI: 10.1111/gcb.14773

The Crux

Most of us know that climate change will bring warmer, shorter winters to most parts of the world. For many species in areas like the Arctic, it would be easy to interpret this as a good thing – plants grow earlier, so animals get more food, right? Naturally it’s never that simple. Many herbivorous species have evolved in sync with climate cycles so that their reproduction peaks when food becomes available. If season start dates change, these species may not be able to change their own cycles in time. Additionally, what happens if populations of their predators suddenly boom?

Today’s authors wanted to know what role a warming climate played in the population fluctuations of migratory barnacle geese (Branta leucopsis).

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Behavioral Ecology for the Masses

Image Credit: Thomas Haaland, CC BY-SA 2.0, Image Cropped

Previously on this site I’ve summarised an article I wrote linking some concepts from behavioral ecology with evolutionary biology. Now I’m back to say a bit more about what behavioral ecology actually is. Being introduced to the field of behavioral ecology was what sparked me to actually consider a career in science. It’s a fascinating and beautiful field, and I hope I can show you why!

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